Composite

Part:BBa_K5269027

Designed by: Yuichiro Nakai   Group: iGEM24_TokyoTech   (2024-09-28)


INPNC-SpyCatcher

This sequence encodes INPNC and SpyCatcher. This enables surface display on the cell membrane utilizing the SpyCatcher-SpyTag system. Also, This allows the protein to act stably on the Membrane Vesicle, allowing for efficient shRNA introduction and the ability to remain on the plant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 481
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 429
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None